Generation and characterization of {beta}1,2-gluco-oligosaccharide probes from Brucella abortus cyclic {beta}-glucan and their recognition by C-type lectins of the immune system

We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the β1,2-gluco-oligosaccharides, with degrees of polymerization 2–13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses, we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the β1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CβG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by β1,2-glucans in mammalian systems.

Source: Glycobiology - October 18, 2016 Category: Biology Authors: Zhang, H., Palma, A. S., Zhang, Y., Childs, R. A., Liu, Y., Mitchell, D. A., Guidolin, L. S., Weigel, W., Mulloy, B., Ciocchini, A. E., Feizi, T., Chai, W. Tags: Original articles Source Type: research