V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog A Synthetic Modified mRNA Drives Reprogramming of Human Pancreatic Duct-Derived Cells Into Insulin-Secreting Cells

This study shows an innovative and robust in vitro system for large-scale production of β-like cells from human pancreatic duct-derived cells (HDDCs) using a nonintegrative RNA-based reprogramming technique. V-Maf musculoaponeurotic fibrosarcoma oncogene homolog A overexpression was efficient and sufficient to induce β-cell differentiation and insulin secretion from HDDCs in response to glucose stimulation, allowing the cells to mitigate hyperglycemia in diabetic SCID-beige mice. The data describe a new, reliable, and fast procedure in adult human pancreatic cells to generate clinically relevant amounts of new β cells with the potential to reverse diabetes.

Source: Stem Cells Translational Medicine - October 18, 2016 Category: Stem Cells Authors: Corritore, E., Lee, Y.-S., Pasquale, V., Liberati, D., Hsu, M.-J., Lombard, C. A., Van Der Smissen, P., Vetere, A., Bonner-Weir, S., Piemonti, L., Sokal, E., Lysy, P. A. Tags: Adult Stem Cells, Pancreas, Enabling Technologies for Cell-Based Clinical Translation Source Type: research