Generation of a transgenic medaka (Oryzias latipes) strain for visualization of nuclear dynamics in early developmental stages

In conclusion, the aNLS possesses a strong nuclear localization activity and is a useful probe for fluorescent observation of the pronuclei and nuclei in early developmental stage of medaka.

This is the first study to label pronuclei in the living fish embryo. We used artificially designated nuclear localization signal (aNLS) fused with GFP protein as a reporter. This aNLS brings the reporter protein into pronuclear before the fusion of male and female pronuclears in living embryo.

Source: Development, Growth and Differentiation - September 30, 2016 Category: Research Authors: Takanobu Inoue, Atsuo Iida, Shingo Maegawa, Atsuko Sehara ‐Fujisawa, Masato Kinoshita Tags: Original Article Source Type: research