Platelet-Activating Factor Quantification Using Reversed Phase Liquid Chromatography and Selected Reaction Monitoring in Negative Ion Mode

This study elucidates a facile, accurate liquid chromatography –mass spectrometry analytical method for PAF. In negative ion mode using electrospray ionization, collisionally-activated dissociation analysis showed a unique product ion for acetate adducts of PAF molecular species representing the loss of methyl acetate from the polar head group and loss of a p art of the acetate group from thesn-2 position. This product ion was exploited for selected reaction monitoring of PAF molecular species following separation by reversed-phase liquid chromatography. Standard calibration responses were determined, and this method was able to detect as low as 100  fmol of PAF. Finally, PAF molecular species were quantified in human neutrophils and monocytes.

Source: Lipids - October 17, 2016 Category: Lipidology Source Type: research