Transcriptional profiles of WNV neurovirulence in a genetically diverse Collaborative Cross population

Publication date: Available online 14 October 2016 Source:Genomics Data Author(s): Richard Green, Courtney Wilkins, Sunil Thomas, Aimee Sekine, Renee C. Ireton, Martin T. Ferris, Duncan M. Hendrick, Kathleen Voss, Fernando Pardo-Manuel de Villena, Ralph S. Baric, Mark T. Heise, Michael Gale West Nile Virus (WNV) is a mosquito-transmitted virus from the Flaviviridae family that causes fever in 1 in 5 infected people. WNV can also become neuro-invasive and cross the blood-brain barrier leading to severe neurological symptoms in a subset of WNV infected individuals Loraine et al. (2015) . WNV neuro-invasion is believed to be influenced by a number of factors including host genetics. In order to explore these effects and recapitulate the complex immune genetic differences among individuals, we studied gene expression following WNV infection in the Collaborative Cross (CC) model. The CC is a mouse genetics resource composed of >70 independently bred, octo-parental recombinant inbred mouse lines Collaborative Cross Consortium (2012 Feb) [2]. To identify the individual host gene expression signatures influencing protection or susceptibility to WNV disease and WNV neuroinvasion, we used the nanostring nsolver platform to quantify gene expression in brain tissue isolated from WNV-infected CC mice at days 4, 7 and 12 post-infection Geiss et al. (2008 Mar) [3]. This nanostring technology provided a high throughput, non-amplification based mRNA quantitation method to...

Source: Genomics Data - October 13, 2016 Category: Genetics & Stem Cells Source Type: research