Identifying protective host gene expression signatures within the spleen during West Nile virus infection in the collaborative cross model

Publication date: Available online 14 October 2016 Source:Genomics Data Author(s): Richard Green, Courtney Wilkins, Sunil Thomas, Aimee Sekine, Renee C. Ireton, Martin T. Ferris, Duncan M. Hendrick, Kathleen Voss, Fernando Pardo-Manuel de Villena, Ralph Baric, Mark Heise, Michael Gale Flaviviruses are hematophagous arthropod-viruses that pose global challenges to human health. Like Zika virus, West Nile Virus (WNV) is a flavivirus for which no approved vaccine exists Loraine et al. (2015) . The role host genetics play in early detection and response to WNV still remains largely unexplained. In order to capture the impact of genetic variation on innate immune responses, we studied gene expression following WNV infection using the collaborative cross (CC). The CC is a mouse genetics resource composed of hundreds of independently bred, octo-parental recombinant inbred mouse lines Collaborative Cross Consortium (2012 Feb) [2]. To accurately capture the host immune gene expression signatures of West Nile infection, we used the nanostring platform to evaluate gene expression in spleen tissue isolated from CC mice infected with WNV over a time course of 4, 7, and 12days' post-infection Geiss et al. (2008 Mar) [3]. Nanostring is a non-amplification based digital method to quantitate gene expression that uses color-coded molecular barcodes to detect hundreds of transcripts in a sample. Using this approach, we identified unique gene signatures in spleen tissue at days 4, ...

Source: Genomics Data - October 13, 2016 Category: Genetics & Stem Cells Source Type: research