Effects of domain composition on catalytic activity of human UDP-glucose:glycoprotein glucosyltransferases

In this study, we aimed to clarify the contribution of the noncatalytic domains by comparing activities of truncated forms of recombinant HUGT1/HUGT2 and HUGT1/HUGT2 chimeras with full-length enzymes. Our results obtained by using synthetic substrate indicate that the C-terminal catalytic regions of HUGTs are functional as UGGT. While the activity of HUGT1, but not that of HUGT2, was enhanced by the presence of N-terminal domains, activities of catalytic domains are similar between two homologs.

Source: Glycobiology - September 30, 2016 Category: Biology Authors: Takeda, Y., Seko, A., Fujikawa, K., Izumi, M., Kajihara, Y., Ito, Y. Tags: Glycan Recognition Source Type: research