Bovine annulus fibrosus cell lines isolated from intervertebral discs

Publication date: Available online 3 October 2016 Source:Genomics Data Author(s): Petra Kraus, Thomas Lufkin The adult bovine (Bos taurus) intervertebral disc is primarily comprised of two major tissue types: The outer annulus fibrosus (AF) and the central nucleus pulposus (NP). We isolated several primary cell lineages of passage (P) 0 cells from the AF tissue omitting typically used enzymatic tissue digestion protocols. The cells grow past p10 without signs of senescence in DMEM+10% FCS on 0.1% gelatin coated/uncoated surfaces of standard cell culture plates and survive freeze-thawing. Preliminary analysis of the AF derived cells for expression of the two structural genes Col1a1 and Col2a1 was performed by PISH recapitulating the expression observed in vivo.

Source: Genomics Data - October 2, 2016 Category: Genetics & Stem Cells Source Type: research