Photocontrol of AMPA Receptors with a Photochromic Ligand

Photochromic ligands (PCLs), recently introduced by our group as a tool for researchers in neuroscience, offer the ability to control native receptors with light in a reversible fashion without the need for any genetic manipulation. Here we describe the application of the PCL Azo-Tetrazole-AMPA-3 (ATA-3) to reversibly gate native AMPA-receptors with blue light and thereby control the activity of cortical neurons in brain slices.

http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-4939-0470-9_5

Source: Springer protocols feed by Protein Science - April 11, 2014 Category: Biochemistry Source Type: news