Photoswitching of Cell Surface Receptors Using Tethered Ligands

We describe here the design of light-gated ionotropic and metabotropic glutamate receptors, the selection of a site for cysteine placement, the method for PTL attachment, and a detailed protocol of photoswitching experiments in cultured cells. These descriptions can guide applications of Chemical Optogenetics to other receptors and serve as a starting point for use in more complex preparations.
Source: Springer protocols feed by Protein Science - Category: Biochemistry Source Type: news