A haploid HSV ‐1 genome platform for vector development: testing of the tetracycline‐responsive switch shows interference by ICP0

ConclusionsOur new haploid HSV‐1 BAC is a useful platform for building multiply deleted HSV‐1 vectors. Deletion of the ICP34.5 gene in this backbone renders viral growth dependent on ICP0, but ICP0 expression could not be regulated by tet‐responsive transcriptional regulators.

Source: The Journal of Gene Medicine - August 31, 2016 Category: Genetics & Stem Cells Authors: Hena Khalique, Jorge L ópez Marco, Filip Lim Tags: RESEARCH ARTICLE Source Type: research