Enantioselective acetylation of (R,S)-atenolol: The use of Candida rugosa lipases immobilized onto magnetic chitosan nanoparticles in enzyme-catalyzed biotransformation

Publication date: December 2016 Source:Journal of Molecular Catalysis B: Enzymatic, Volume 134, Part A Author(s): Adam Sikora, Dorota Chełminiak-Dudkiewicz, Tomasz Siódmiak, Agata Tarczykowska, Wiktor Dariusz Sroka, Marta Ziegler-Borowska, Michał Piotr Marszałł This paper describes the enzyme immobilization protocol as well as the enzymatic method for the direct resolution of (R,S)-atenolol. The used magnetic enzyme carriers possess on their surface new-synthetized chitosan derivatives with free amine groups distanced by ethyl or butyl chain. Additionally the catalytic activity of two types of commercially available lipases from Candida rugosa immobilized onto two different magnetic nanoparticles were compared. The highest values of enantioselectivity (E=66.9), enantiomeric excess of product (eep =94.1%) and conversion (c=41.84%) were obtained by using lipase from Candida rugosa OF immobilized onto Fe3O4-CS-EtNH2. The study confirmed that even after 5 reaction cycles the immobilized lipase maintain its high catalytic activity. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research