Reproducibility of Uniform Spheroid Formation in 384-Well Plates: The Effect of Medium Evaporation

In this study, we examined the effect of evaporation on the reproducibility of spheroids of tumor and nontumor cell lines in 384-well plates, and show that culture conditions that prevent evaporation-induced medium loss result in the formation of uniform spheroids across the plate. Additionally, we also present a few technical improvements to increase the scalability of the liquid-overlay spheroid culturing technique in MPs, together with a simple software routine for the quantification of spheroid size. We believe that these cost-effective improvements will aid in further improvement of spheroid cultures for HTS drug discovery.
Source: Journal of Biomolecular Screening - Category: Molecular Biology Authors: Tags: Special Collection Source Type: research