Novel long non-coding RNA GACAT3 promotes gastric cancer cell proliferation through the IL-6/STAT3 signaling pathway

In this study, to investigate its function, we synthesized small interference RNAs (siRNAs) against GACTA3 and developed a GACAT3 overexpression vector (pcDNA3-GACAT3), respectively. The siRNA-mediated knockdown ofGACAT3 significantly decreased cell proliferation of the gastric cancer HGC-27 cells, in whichGACAT3 is overexpressed. Furthermore,GACAT3 overexpression in gastric cancer SGC-7901 cells promoted cell growth. Moreover,GACAT3 expression in HGC-27 cells was greatly upregulated by IL-6 treatment in a concentration-dependent manner. In contrast, siRNA-mediated knockdown ofSTAT3 decreasedGACAT3 expression even in the presence of IL-6. These results demonstrated that as a downstream target of the  IL6/STAT3 signaling, lncRNAGACAT3 promotes gastric cancer cell growth suggesting thatGACAT3 is an inflammatory response gene and may be served as a valuable potential target for the treatment of gastric cancer.
Source: Tumor Biology - Category: Cancer & Oncology Source Type: research