Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media

Publication date: Available online 15 September 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Elena Fernández-Fueyo, Yan Ni, Alvaro Gomez Baraibar, Miguel Alcalde, Lukas M. van Langen, Frank Hollmann The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and tertBuOOH as oxidant. Stability and activity issues still have to be addressed. Nevertheless, gram-scale production of enantiopure (R)-1-phenylethanol was achieved with respectable 90000 turnovers of the biocatalyst. Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - September 15, 2016 Category: Biochemistry Source Type: research