In vivo and ex vivo 19 ‐fluorine magnetic resonance imaging and spectroscopy of beta‐cells and pancreatic islets using GLUT‐2 specific contrast agents

In this study, we confirmed that, similar to DMH, 19FMHs inhibit insulin secretion and increase the blood glucose level in mice temporarily (approximately two hours). We were able to assess the distribution of 19FMHs in vivo with a temporal resolution of about 20 minutes, which showed a quick removal of 19FMH from the circulation (within two hours). Ex vivo MR spectroscopy confirmed a preferential uptake of 19FMH in tissue with high expression of the GLUT‐2 transporter, such as liver, endocrine pancreas and kidney. No indication of further metabolism was found. In summary, 19FMHs are potentially suitable for visualizing and tracking of GLUT‐2 expressed cells. However, current bottlenecks of this technique related to the quick clearance of the compound and relative low sensitivity of 19F MRI need to be overcome. Copyright © 2016 John Wiley & Sons, Ltd. Ex vivo 19F MR spectroscopy confirms preferential uptake of 19F fluoro‐deoxy‐mannoheptuloses in tissue with high expression of the GLUT‐2 transporter, such as liver, endocrine pancreas and kidney. However, in vivo 19F MRI was not sensitive enough for non‐invasive detection within reasonable scan time.
Source: Contrast Media and Molecular Imaging - Category: Radiology Authors: Tags: Full paper Source Type: research