Tannerella forsythia GroEL induces inflammatory bone resorption and synergizes with interleukin ‐17

This study investigated the potential of T. forsythia GroEL to induce inflammatory bone resorption and examined the cooperative effect of IL‐17 and T. forsythia GroEL on inflammatory responses. Human gingival fibroblasts (HGFs) and periodontal ligament (PDL) fibroblasts were stimulated with T. forsythia GroEL and/or IL‐17. Gene expression of IL‐6, IL‐8, and cyclooxygenase‐2 (COX‐2) and concentrations of IL‐6, IL‐8, and prostaglandin E2 (PGE2) were measured by real‐time reverse transcription polymerase chain reaction and enzyme‐linked immunosorbent assays, respectively. After stimulation of MG63 cells with T. forsythia GroEL and/or IL‐17, gene expression of osteoprotegerin (OPG) was examined. After subcutaneous injection of T. forsythia GroEL and/or IL‐17 above the calvaria of BALB/c mice, calvarial bone resorption was assessed by micro‐computed tomography and histological examination. Tannerella forsythia GroEL induced IL‐6 and IL‐8 production in HGFs and PDL cells, and IL‐17 further promoted IL‐6 and IL‐8 production. Both T. forsythia GroEL and IL‐17 synergistically increased PGE2 production and inhibited OPG gene expression. Calvarial bone resorption was induced by T. forsythia GroEL injection, and simultaneous injection of T. forsythia GroEL and IL‐17 further increased bone resorption. These results suggest that T. forsythia GroEL is a novel virulence factor that can contribute to inflammatory bone resorption caused by T. ...
Source: Oral Microbiology and Immunology - Category: Microbiology Authors: Tags: Original Article Source Type: research