Genome wide binding (ChIP-seq) of murine Bapx1 and Sox9 proteins in vivo and in vitro

Publication date: Available online 8 September 2016 Source:Genomics Data Author(s): Sumantra Chatterjee, Petra Kraus, V. Sivakamasundari, Sook Peng Yap, Vibhor Kumar, Shyam Prabhakar, Thomas Lufkin This work pertains to GEO submission GSE36672, in vivo and in vitro genome wide binding (ChIP-Seq) of Bapx1 and Sox9 proteins. We have previously shown that transcriptional profiling combined with genome wide binding data is a powerful means to elucidate the molecular mechanism behind cell type specification and the generation of tissues and subsequent organs Chatterjee et al. (2014) . In our previously published work we revealed the function of the homeobox gene Bapx1 during the embryogenesis of five distinct at a relevant developmental stage (E12.5), microarray analysis of EGFP+ wildtype and EGFP+ mutant cells was compared in conjunction with ChIP-Seq analysis of Bapx1 and Sox9 proteins in vivo. We also analyzed the development of the vertebral column by comparing microarray and ChIP-Seq data for Bapx1 with similarly generated data sets for Sox9 to generate a regulatory network controlling various aspects of murine embryonic development.

Source: Genomics Data - September 8, 2016 Category: Genetics & Stem Cells Source Type: research