Trans 10, cis 15 18:2 Isolated from Beef Fat Does Not Have the Same Anti-Adipogenic Properties as Trans 10, cis 12 –18:2 in 3T3-L1 Adipocytes

AbstractDuring ruminal biohydrogenation of α-linolenic acid, a non-conjugated non-methylene interrupted dienoic acid is formed containing at10 double bond, namelyt10,c15 –18:2. The present study was designed to examine whethert10,c15 –18:2 would exert similar anti-adipogenic effects compared tot10,c12 –18:2 in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were treated with 35 or 70 µM of LNA,t10,c12 –18:2,t10,c15 –18:2, or bovine serum albumin (BSA) vehicle control for 120 h. Cellular triacylglycerol and protein were quantified using commercial colorimetric kits. Cells were analyzed for fatty acid composition and gene expression using gas chromatography and quantitative PCR, respectively.Trans10,cis12 –18:2 decreased (P <  0.05) the adipocyte triacylglycerol (TAG) content, which was mainly related to a reduction in saturated fatty acids (SFA; e.g., 16:0 and 15:0) andcis monounsaturated fatty acids (c-MUFA; e.g.,c9 –16:1 andc9 –18:1).Trans10,cis12 also decreased (P <  0.05) the expression of genes related to fatty acid synthesis (ACACA,FASN), delta-9 desaturation (SCD1), fatty acid elongation (ELOVL5), and fatty acid uptake (LPL) and upregulated (P <  0.05) the expression of the rate-liming enzyme involved in fatty acid β-oxidation (CPT1). In contrast, LNA andt10,c15 –18:2 did not affect the gene expression and cellular content of the TAG, SFA,c-MUFA, orSCD1 indices in adipocytes. Our findings suggest thatt10,c15 –18:2, despite hav...

Source: Lipids - September 2, 2016 Category: Lipidology Source Type: research