Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B

Conclusions PLA with either qPCR or dPCR readout have potential as new diagnostic applications for the detection of pathogens where nucleic acid based tests do not indicate viability or expression of toxins. Importantly, since it is not always necessary to use two different antibodies, the pool of potential antibodies useful for PLA diagnostic assays is usefully enhanced.
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research