Mutational analysis of structural elements in a class-I cyclic di-GMP riboswitch to elucidate its regulatory mechanism

In this study, we performed in vivo reporter assays and in vitro biochemical analyses of the full-length riboswitch and its aptamer domain. We evaluated the results of in vivo and in vitro analyses to elucidate the regulatory mechanism of the Vc2 riboswitch. The present results suggest that recognition of c-di-GMP ligand by the Vc2 riboswitch aptamer domain downregulates expression of its downstream ORF primarily at the translational level.

Source: Journal of Biochemistry - August 25, 2016 Category: Biochemistry Authors: Inuzuka, S., Nishimura, K.-I., Kakizawa, H., Fujita, Y., Furuta, H., Matsumura, S., Ikawa, Y. Tags: Regular Papers Source Type: research