Significance of immunohistochemistry and DNA ploidy in differential diagnosis of flat urothelial lesions and conditions using CK20, p53, CD44, ErbB-2, and image cytometry

Conclusion: In conclusion, a panel of CK20, p53, CD44, and ErbB-2 helps to differentiate urothelial CIS from non-neoplastic urothelial atypias. Positive staining for at least two of the three antibodies (CK20, p53, and ErbB-2) is strongly associated with CIS. DNA aneuploidy helps in differentiating CIS from reactive atypia. However, the histologic findings should be a primary determinant in the diagnosis of flat urothelial lesions, with IHC and DNA ploidy playing a supportive confirmatory role.
Source: Egyptian Journal of Pathology - Category: Pathology Tags: Original Articles Source Type: research