Type III Secretion System of Pseudomonas aeruginosa Affects Matrix Metalloproteinase 12 (MMP-12) and MMP-13 Expression via Nuclear Factor {kappa}B Signaling in Human Carcinoma Epithelial Cells and a Pneumonia Mouse Model

We examined the effects of ExoS, ExoT, and ExsA:: on MMP-12 and MMP-13 production in NCI-H292 cells. ExoS and ExoT markedly decreased the neutrophil count in bronchoalveolar lavage fluid, with a reduction in proinflammatory mediators, MMP-12, and MMP-13. ExoS and ExoT reduced NF-B phosphorylation, together with MMP-12 and MMP-13 expression in PAK-infected mouse models and NCI-H292 cells. To conclude, P. aeruginosa infection induced the expression of MMPs, and P. aeruginosa T3SS appeared to be a key player in MMP-12 and MMP-13 expression, which is further controlled by NF-B signaling. These findings might be useful in devising a novel therapeutic approach to chronic pulmonary infections that involves decreasing the ExoS and ExoT levels.

Source: The Journal of Infectious Diseases - August 23, 2016 Category: Infectious Diseases Authors: Park, J.-W., Kim, Y.-J., Shin, I.-S., Kwon, O.-K., Hong, J. M., Shin, N.-R., Oh, S.-R., Ha, U.-H., Kim, J.-H., Ahn, K.-S. Tags: PATHOGENESIS AND HOST RESPONSE Source Type: research