111In-Labeled Trastuzumab-Modified Gold Nanoparticles are Cytotoxic In Vitro to HER2-Positive Breast Cancer Cells and Arrest Tumor Growth In Vivo in Athymic Mice After Intratumoral Injection

Gold nanoparticles (AuNP; 30nm) were modified with polyethylene glycol (PEG) chains linked to trastuzumab for binding to HER2-positive breast cancer (BC) cells and diethylenetriaminepentaacetic acid (DTPA) for complexing the Auger electron-emitter, 111In (trastuzumab-AuNP-111In). Our objective was to determine the cytotoxicity of trastuzumab-AuNP-111In on HER2-positive BC cells in vitro and evaluate its tumor growth-inhibition properties and normal tissue toxicity in vivo following intratumoral (i.t.) injection in mice with s.c.
Source: Nuclear Medicine and Biology - Category: Nuclear Medicine Authors: Source Type: research