Applying CRISPR –Cas9 tools to identify and characterize transcriptional enhancers

Nature Reviews Molecular Cell Biology 17, 597 (2016). doi:10.1038/nrm.2016.79 Authors: Rui Lopes, Gozde Korkmaz & Reuven Agami The development of the CRISPR–Cas9 system triggered a revolution in the field of genome engineering. Initially, the use of this system was focused on the study of protein-coding genes but, recently, a number of CRISPR–Cas9-based tools have been developed to study non-coding transcriptional regulatory elements.
Source: Nature Reviews Molecular Cell Biology - Category: Cytology Authors: Tags: Perspectives Source Type: research