Detection of blaKPC and blaGES carbapenemase genes in Klebsiella pneumoniae isolated from hospitalized patients in Kashan, Iran.

In this study we have detected the blaKPC and blaGES carbapenemase genes and integron class 1 in Klebsiella pneumoniae isolated from hospitalized patient in Kashan, Iran. In a cross-sectional study, a total of 181 K. pneumoniae isolates were recovered K. pneumoniae from clinical specimens during November 2013 to October 2014. Antimicrobial susceptibility profiles were determined using disk diffusion method according to CLSI guidelines. Carbapenem-resistant K. pneumoniae isolates were identified. PCR method and sequencing were used for detection of integron class 1 and blaKPC and blaGES carbapenemase genes. Of the 181 K. pneumoniae isolates, 150 (82.9%) identified as MDR strains. Among carbapenems, the most resistance rates were seen against imipenem 36 (19.9%) using disk diffusion method. All MDR K. pneumoniae isolates carried integron class 1 and 21 (11.6%) and 42 (23.2%) of K. pneumoniae isolates carried blaKPC and blaGES genes, respectively. The data of this investigation revealed that the prevalence of GES type carbapenemase was higher than KPC type among clinical isolates of K. pneumoniae in Kashan, Iran. Also presence of integron class 1 in all isolates highlight the risk of rapid spread of resistance genes especially in clinical settings. PMID: 27527726 [PubMed - as supplied by publisher]
Source: Recent Patents on Anti-Infective Drug Discovery - Category: Infectious Diseases Tags: Recent Pat Antiinfect Drug Discov Source Type: research