Diagnostic utility of epithelial and melanocitic markers with double sequential immunohistochemical staining in differentiating melanoma in situ from invasive melanoma

Identification of melanoma in situ and its distinction from invasive melanoma is important due to its significant impact on morbidity and mortality. However, This interpretation can cause pitfalls in the diagnosis even with the use of immunohistochemistry. The aim of this study is to evaluate the diagnostic utility of epithelial makers (AE1/AE3, CK5/6 and p63) combined with melanocytic markers (HMB-45 S-100 or Melan A) using dual-color immunohistochemical staining, performed on a single slide by sequentially applying the antibodies.
Source: Annals of Diagnostic Pathology - Category: Pathology Authors: Source Type: research