Monocyty z ekspresj ą Tie-2 u chorych na przewlekłą białaczkę limfocytową

Publication date: Available online 16 August 2016 Source:Acta Haematologica Polonica Author(s): Agnieszka Bojarska-Junak, Piotr Grundszok, Małgorzata Waldowska, Justyna Woś, Sylwia Chocholska, Iwona Hus, Wioleta Kowalska, Katarzyna Gęca, Waldemar Tomczak, Jacek Roliński Introduction In peripheral blood, monocytes form a heterogeneous population of cells. One particular subset of circulating monocytes is expressing the angiopoietin receptor Tie-2 (Tie2-expressing monocyte; TEM). TEM are characterized by tumor promoting properties. However, the role of TEM in chronic lymphocytic leukemia (CLL) immunopathogenesis remains undefined. Material and Methods Here, we evaluated the monocytes with Tie-2 expression (CD14+Tie-2+) in peripheral blood of CLL patients (n=55) and normal subjects (n=15) by flow cytometry. We investigated possible associations between TEM and poor prognostic factors such as CD38 or ZAP-70 expression, Rai stage and unfavorable cytogenetic abnormalities. Moreover, we investigated the association of TEM percentage with CD14++CD16+ monocytes and Treg percentages. Results We found that CLL patients had a higher percentage of CD14+Tie-2+ monocytes compared to normal controls. The percentage of TEM was positively associated with ZAP-70 expression as well as with unfavourable cytogenetic changes: del(17p) and/or del(11q). The frequency of TEM increased with the disease stage. We showed no correlation between the percentage of TEM and CD38 expression. ...
Source: Acta Haematologica Polonica - Category: Hematology Source Type: research