Tumour necrosis factor ‐alpha (‐308G/A) promoter polymorphism is associated with ulcerative colitis in Brazilian patients

This study aimed to analyse the gene polymorphisms in Brazilian patients with inflammatory bowel disease. A total of 101 patients diagnosed with inflammatory bowel disease were analysed for the tumour necrosis factor‐alpha (‐308 G/A; rs1800629) and interleukin‐10 (‐1082 G/A; rs1800896) gene polymorphisms. Genotyping was performed through polymerase chain reaction–sequence‐specific primer, then fractionated on 2% agarose gel and visualized after staining by ethidium bromide. The anatomic–clinical form of Crohn's disease (CD) predominant was the inflammatory (32.75%), followed by fistulizing (29.31%) and 27.58% stricturing. As control group, a total of 136 healthy subjects, from the same geographical region, were enrolled. The statistical analyses were performed using R program. The frequency of the A allele at tumour necrosis factor‐alpha was high in ulcerative colitis (UC) patients (51%) than in controls (22%; P > 0.01). No statistical difference was found with the genotypic and allelic frequencies of CD patients compared to controls (P = 0.54). The polymorphism ‐1082G/A of interleukin‐10 was not statistical different between the diseases compared to controls. Tumour necrosis factor‐alpha (TNF‐α) (‐308G/A) is associated with UC onset, suggesting that the presence of ‐308A allele could confer a relative risk of 3.62 more to develop UC in general population. Further studies, increasing the number of individuals, should be performed to ratif...
Source: International Journal of Immunogenetics - Category: Genetics & Stem Cells Authors: Tags: Original Article Source Type: research