Tissue specific regulation of sirtuin and nicotinamide adenine dinucleotide biosynthetic pathways identified in C57Bl/6 mice in response to high-fat feeding

The sirtuin/nicotinamide adenine dinucleotide (NAD) system is implicated in development of type 2 diabetes (T2D) and diet-induced obesity, a major risk factor for T2D. Mechanistic links have not yet been defined. Sirtuin/NAD system gene expression and NAD/NADH levels were measured in liver, white adipose tissue (WAT) and skeletal muscle from mice fed either a low-fat diet (LFD) or high-fat diet (HFD) for 3 days up to 16 weeks. An in-house custom designed multiplex gene expression assay, assessed all 7 mouse sirtuins (SIRT1 –7) and 16 enzymes involved in conversion of tryptophan, niacin, nicotinamide riboside and metabolic precursors to NAD.
Source: The Journal of Nutritional Biochemistry - Category: Biochemistry Authors: Source Type: research