Next-generation sequencing analysis of off-ladder alleles due to migration shift caused by sequence variation at D12S391 locus

Short tandem repeat (STR) analysis is widely used for population genetics, kinship analysis, and forensic examinations [1]. In STR analysis, length polymorphisms at target loci are usually amplified in a multiplex polymerase chain reaction (PCR) and detected by capillary electrophoresis (CE) under denaturing conditions. It was reported that some STR loci have sequence variation in the repeat region and have alleles of the same length but with different internal sequences (now called isoalleles) [2].

Source: Legal Medicine - August 11, 2016 Category: Forensic Medicine Authors: Koji Fujii, Haruhiko Watahiki, Yusuke Mita, Yasuki Iwashima, Hajime Miyaguchi, Tetsushi Kitayama, Hiroaki Nakahara, Natsuko Mizuno, Kazumasa Sekiguchi Source Type: research