A miRNA-target network putatively involved in follicular atresia

In a previous microarray study we identified a subset of miRNAs which expression was distinctly higher in atretic than healthy follicles of cattle. In the present study we investigated the involvement of those miRNAs in granulosa and theca cells during atresia. RT-qPCR confirmed that miR-21-5p/-3p, miR-150, miR-409a, miR-142-5p, miR-378, miR-222, miR-155 and miR-199a-5p were expressed at higher levels in atretic than healthy follicles (9-17 mm, classified based on steroidogenic capacity). All miRNAs except miR-21-3p and miR-378 were expressed at higher levels in theca than granulosa cells.

Source: Domestic Animal Endocrinology - August 12, 2016 Category: Endocrinology Authors: F.X. Donadeu, J. Ioannidis Source Type: research