Potent antimicrobial and antibiofilm activities of bacteriogenically synthesized gold-silver nanoparticles against pathogenic bacteria and their physiochemical characterizations

The objective of this study was to develop a bimetallic nanoparticle with enhanced antibacterial activity that would improve the therapeutic efficacy against bacterial biofilms. Bimetallic gold–silver nanoparticles were bacteriogenically synthesized using -proteobacterium, Shewanella oneidensis MR-1. The antibacterial activities of gold–silver nanoparticles were assessed on the planktonic and biofilm phases of individual and mixed multi-cultures of pathogenic Gram negative (Escherichia coli and Pseudomonas aeruginosa) and Gram positive bacteria (Enterococcus faecalis and Staphylococcus aureus), respectively. The minimum inhibitory concentration of gold–silver nanoparticles was 30–50 µM than that of other nanoparticles (>100 µM) for the tested bacteria. Interestingly, gold–silver nanoparticles were more effective in inhibiting bacterial biofilm formation at 10 µM concentration. Both scanning and transmission electron microscopy results further accounted the impact of gold–silver nanoparticles on biocompatibility and bactericidal effect that the small size and bio-organic materials covering on gold–silver nanoparticles improves the internalization and thus caused bacterial inactivation. Thus, bacteriogenically synthesized gold–silver nanoparticles appear to be a promising nanoantibiotic for overcoming the bacterial resistance in the established bacterial biofilms.
Source: Journal of Biomaterials Applications - Category: Materials Science Authors: Tags: Nanotechnology in Biomaterials Source Type: research