Evaluation of antioxidant, antimicrobial and cytotoxic activities of seed crude extracts of Ammi majus grown in Oman

Publication date: Available online 12 August 2016 Source:Egyptian Journal of Basic and Applied Sciences Author(s): Rehab M. Al-Hadhrami, Mohammad A. Hossain Ammi majus has long been used as an herbal medicine in several countries for skin disorders, regulation of menstruation and for conditions in which diuresis is indicated. The present study was undertaken to evaluate the antioxidant, antimicrobial and cytotoxic activities of the crude extracts of locally growing Ammi majus (A. majus). Initially, methanol crude extract was prepared from powdered seed samples of A. majus by applying Soxhlet extraction method. Successive extracts were then derived by suspending methanol free crude extract in water followed by successive extraction with solvents of different polarities in an order of increasing polarity. The evaluations of antioxidant, antimicrobial and cytotoxic activities of seed crude extracts of A. majus were determined by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), agar disc diffusion and Artemia lethality methods, respectively. The highest antioxidant activity was observed in case of chloroform crude extract whereas the lowest activity was corresponding to methanol crude extract. However, the highest IC50 value was obtained with chloroform crude extract but the lowest result was observed in case of ethyl acetate crude extract. All crude extracts of A. majus displayed moderate antimicrobial activity against one Gram positive bacteria, Staphylococcus aureus (S. aureus), and...
Source: Egyptian Journal of Basic and Applied Sciences - Category: Science Source Type: research