Effects of different spacer arms on Cibacron Blue modification and protein affinity adsorption on magnetic microspheres

Publication date: Available online 11 August 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Dong-Hao Zhang, Na Chen, Mei-Na Yang, Yi-Fang Dou, Jie Sun, Ya-Dong Liu, Gao-Ying Zhi In order to obtain a deep insight into the effects of spacer arms on performing CB modification and dye-affinity adsorption, poly(methyl methacrylate) (PMMA) magnetic microspheres were prepared and modified respectively with poly(ethylene glycol) (PEG), poly(vinyl alcohol) (PVA) and ethylenediamine (EDA) as spacer arms. Then, an affinity dye-ligand, Cibacron Blue F3GA (CB), was coupled with these spacer arms to synthesize three kinds of CB-attached magnetic microsphere including CB-PEG-PMMA, CB-PVA-PMMA and CB-EDA-PMMA. CB density of the resulting microspheres was determined to be 138.1μmol/g for CB-PEG-PMMA, 209.7μmol/g for CB-PVA-PMMA, and 266.0μmol/g for CB-EDA-PMMA, respectively. The affinity adsorption performances of the resulting microspheres were evaluated using bovine serum albumin (BSA). The results showed that the adsorption capacity of BSA increased with the increase in CB density, and CB-EDA-PMMA microspheres exhibited the highest adsorption capacity. Furthermore, the effects of pH, BSA concentration and ionic strength on BSA adsorption were investigated and the maximum adsorption capacity was found to be 114.0mg/g CB-EDA-PMMA microspheres. Moreover, the reusability of the microspheres was also studied. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research