Effects of in vitro fermentation of barley β‐glucan and sugar beet pectin using human fecal inocula on cytokine expression by dendritic cells

ConclusionsThe fermentation of the two fibers led to fiber‐specific amounts of SCFA, glycosidic metabolites, and different immunomodulatory properties. BMDC from TLR2/4 knockout mice did not respond to the digest microbiota and SCFA, making it a useful approach to study temporal effects of fermentation on the immunomodulatory effects of fibers.

Dietary fibres influence the immune system. This stimulation by dietary fibres might be influenced by present endotoxins such as LPS. By using bone marrow derived dendritic cells (BMDCs) from TLR2/4 knockout mice, this undesired stimulation was circumvented and controls containing only microbiota did not cause any TNF induction in the BMDCs. In contrast, samples containing fibres (e.g. β‐glucan (left) or sugar beet pectin (right)) induced immune response in different ways depending on the fibres. In addition, glycosidic fibre degradation products induced even higher amounts of TNF, depending on the fermentation stage (0–48 hours).

Source: Nahrung / Food - August 10, 2016 Category: Nutrition Authors: Christiane R ösch, Nico Taverne, Koen Venema, Harry Gruppen, Jerry M. Wells, Henk A. Schols Tags: Research Article Source Type: research