M1 polarization and the effect of PGE2 on TNF ‐α production by lymph node cells from dogs with visceral leishmaniasis

This study aimed to evaluate the arginase activity in adherent macrophages cultured from the lymph nodes of healthy and naturally infected dogs and to examine the NO2 and PGE2 levels in the supernatant of these cultures. In addition, the regulatory effect of PGE2 on the production of tumor necrosis factor (TNF‐α) and interleukin‐10 (IL‐10) in supernatants from the total lymph node were observed in leukocyte cultures. The arginase activity was lower in the adherent macrophages cultured from the lymph nodes of naturally infected dogs and there were higher concentrations of NO2 and PGE2 in the supernatants of these cultures. Higher TNF‐α and IL‐10 concentrations were observed in supernatants from total lymph node leukocytes cultures, from infected dogs and the presence of indomethacin only decreased TNF‐α in the supernatant of these cultures. We conclude that the low arginase activity in macrophages suggested that M1 polarization and PGE2 were participating in the immune response and were increasing TNF‐α in CVL.
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Source: Parasite Immunology - July 31, 2016 Category: Parasitology Authors: Gabriela Lovizutto Venturin, Vanessa Marim Chiku, Kathlenn Liezbeth Oliveira Silva, Breno Fernando Martins de Almeida, Val éria Marçal Felix de Lima Tags: Brief Definitive Report Source Type: research