Tracking adenovirus genomes identifies morphologically distinct late DNA replication compartments

In conclusion, our work demonstrates the formation of previously uncharacterized viral DNA replication compartments specific for late phases of infection that produce progeny viral genomes accumulating in ViPR bodies. The spatial relationship between early adenoviral genome replication and subsequent viral gene expression has been established. In contrast when and where newly replicated genomes are linked to progeny virion production remains unclear. Using direct genome‐labeling techniques, we show the formation of a novel subnuclear compartment late in infection, which we named ViPR body that functions as a reservoir for replicated adenoviral genomes, and identified a host factor specifically localizing in the domain. The potential role of ViPR bodies is discussed.
Source: Traffic - Category: Research Authors: Tags: Original Article Source Type: research