Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex – a new promising X‐ray contrast agent

The octahedral cluster compound Na2H8[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6] has been shown to be highly radio dense, thus becoming a promising X‐ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD50) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex Hn[{Re6Se8}(P(C2H4CONH2)(C2H4COO)2)6]n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice. Copyright © 2016 John Wiley & Sons, Ltd. Highly hydrophilic X‐ray contrast octahedral cluster complex [{Re6Se8}(P(CH2CH2CONH2)(CH2CH2COO)2)6]10– does not internalize into cells in vitro and demonstrates a high degree of safety after intravenous injection in animals, thus becoming a promising contrast agent for X‐ray‐based imaging techniques such as computed tomography, radiography, and angiography.
Source: Contrast Media and Molecular Imaging - Category: Radiology Authors: Tags: Full paper Source Type: research