Prostaglandin synthesis by the porcine corpus luteum: effect of tumor necrosis factor- α

The porcine corpus luteum (CL) displays delayed sensitivity to PGF-2 α (luteolytic sensitivity, [LS]) until days 12 to 13 of cycle. The control of LS is unknown, but it is temporally associated with macrophage (which secrete TNF-α) infiltration into the CL. Other studies showed that TNF-α induces LS in vitro and that prostaglandins may be involved in this mechani sm. In experiment 1, PGF-2α and PGE secretion by luteal cells (LCs) was measured on days 4 to 14 of the estrous cycle, and the expression of PTGFS/AKR1B1 and PTGES/mPGES-1, by Western blot, before (day 7) vs after (day 13) the onset of LS.

Source: Domestic Animal Endocrinology - July 13, 2016 Category: Endocrinology Authors: J. Chang, S. Frandsen, J.E. Gadsby Source Type: research