Maintenance of Cytosolic Calcium is Crucial to Extend L-Arginine Therapeutic Benefits During Continuous Dosing

The therapeutic benefits associated with short-term L-Arginine supplementation are lost during continuous dosing. AMP-activated protein kinase (AMPK) functional modulation has been correlated with L-Arginine therapeutic effectiveness, and with tolerance development during continuous supplementation. However, the metabolic link that is responsible for AMPK functional modulation during continuous L-Arginine exposure is currently not known. To explore this, we incubated HUVECs for 7 days with 100 μM L-Arginine, in the presence or absence of other agents; and monitored their effects for eNOS function, and on tolerance sparing effects (viz., cellular glucose accumulation, and oxidative stress).

Source: Nutrition Research - July 20, 2016 Category: Nutrition Authors: Srinidi Mohan, Lisa Harding Tags: Communication Source Type: research