Abstract < /h3 > < p class= " a-plus-plus " > Rabbitfish < em class= " a-plus-plus " > Siganus canaliculatus < /em > was the first marine teleost demonstrated to have the ability to biosynthesize C < sub class= " a-plus-plus " > 20 –22 < /sub > long-chain polyunsaturated fatty acid (LC-PUFA) from C < sub class= " a-plus-plus " > 18 < /sub > PUFA precursors, which..."> Abstract < /h3 > < p class= " a-plus-plus " > Rabbitfish < em class= " a-plus-plus " > Siganus canaliculatus < /em > was the first marine teleost demonstrated to have the ability to biosynthesize C < sub class= " a-plus-plus " > 20 –22 < /sub > long-chain polyunsaturated fatty acid (LC-PUFA) from C < sub class= " a-plus-plus " > 18 < /sub > PUFA precursors, which..." /> Abstract < /h3 > < p class= " a-plus-plus " > Rabbitfish < em class= " a-plus-plus " > Siganus canaliculatus < /em > was the first marine teleost demonstrated to have the ability to biosynthesize C < sub class= " a-plus-plus " > 20 –22 < /sub > long-chain polyunsaturated fatty acid (LC-PUFA) from C < sub class= " a-plus-plus " > 18 < /sub > PUFA precursors, which..." />

Cloning and Characterization of Lxr and Srebp1, and Their Potential Roles in Regulation of LC-PUFA Biosynthesis in Rabbitfish Siganus canaliculatus

< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > Rabbitfish < em class= " a-plus-plus " > Siganus canaliculatus < /em > was the first marine teleost demonstrated to have the ability to biosynthesize C < sub class= " a-plus-plus " > 20 –22 < /sub > long-chain polyunsaturated fatty acid (LC-PUFA) from C < sub class= " a-plus-plus " > 18 < /sub > PUFA precursors, which is generally absent or low in marine teleosts. Thus, understanding the molecular basis of LC-PUFA biosynthesis in rabbitfish will contribute to efforts aimed at optimizing LC-PUFA biosynthesis in teleosts, especially marine species. In the present study, the importance of the transcription factors liver X receptor (Lxr) and sterol regulatory element-binding protein 1 (Srebp1) in regulation of LC-PUFA biosynthesis in rabbitfish was investigated. First, full-length cDNA of < em class= " a-plus-plus " > Lxr < /em > and < em class= " a-plus-plus " > Srebp1 < /em > were cloned and characterized. The < em class= " a-plus-plus " > Lxr < /em > mRNA displayed a ubiquitous tissue expression pattern while < em class= " a-plus-plus " > Srebp1 < /em > was highly expressed in eyes, brain and intestine. In rabbitfish primary hepatocytes treated with Lxr agonist T0901317, the expression of < em class= " a-plus-plus " > Lxr < /em > and < em class= " a-plus-plus " > Srebp1 < /em > was activated, accompanied by elevated mRNA levels of Δ4 and Δ6/Δ5 fatty acyl desaturase (Fad), key enzymes of L...
Source: Lipids - Category: Lipidology Source Type: research