Surgical tissue handling methods to optimize ex vivo fluorescence with the activatable optical probe γ‐glutamyl hydroxymethyl rhodamine green

In conclusion, we demonstrate that the method of tissue handling prior to ex vivo imaging with the activatable probe gGlu‐HMRG has a strong influence on the signal derived from the specimen. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. When testing enzyme activatable optical probes such as gGlu‐HMRG on excised specimens it is important to either keep the specimen intact or use it on a cut surface. Rinsing or pressing the specimen is not recommended, as it decreases the intensity of gGlu‐HMRG activation and may even cause false positives if the GGT is relocated outside the tumor location. Thus, the precise method of tissue handling is important for ex vivo testing of enzyme activatable probes.
Source: Contrast Media and Molecular Imaging - Category: Radiology Authors: Tags: Short communication Source Type: research