HyperCEST detection of cucurbit[6]uril in whole blood using an ultrashort saturation Pre ‐pulse train

Xenon based biosensors have the potential to detect and localize biomarkers associated with a wide variety of diseases. The development and nuclear magnetic resonance (NMR) characterization of cage molecules which encapsulate hyperpolarized xenon is imperative for the development of these xenon biosensors. We acquired 129Xe NMR spectra, and magnetic resonance images and a HyperCEST saturation map of cucurbit[6]uril (CB6) in whole bovine blood. We observed a mean HyperCEST depletion of 84% (n = 5) at a concentration of 5 mM and 74% at 2.5 mM. Additionally, we collected these data using a pulsed HyperCEST saturation pre‐pulse train with a SAR of 0.025 W/kg which will minimize any potential RF heating in animal or human tissue. Copyright © 2016 John Wiley & Sons, Ltd.

The xenon MR spectrum shows chemical shifts corresponding to it's binding to red blood cells, dissolved in blood plasma and exchanging with the cucurbit[6]uril (CB6) cage molecule. By applying an RF pulse at the CB6 chemical shift, a HyperCESt effect is produced allowing the detection of the CB6 molecule in blood (right panel).

Source: Contrast Media and Molecular Imaging - April 12, 2016 Category: Radiology Authors: Francis T. Hane, Peter S. Smylie, Tao Li, Julia Ruberto, Krista Dowhos, Iain Ball, Boguslaw Tomanek, Brenton DeBoef, Mitchell S. Albert Tags: Full paper Source Type: research