Kinetic analysis of drug –protein interactions by affinity chromatography

Publication date: October 2015 Source:Drug Discovery Today: Technologies, Volume 17 Author(s): Cong Bi, Sandya Beeram, Zhao Li, Xiwei Zheng, David S. Hage Information on the kinetics of drug–protein interactions is of crucial importance in drug discovery and development. Several methods based on affinity chromatography have been developed in recent years to examine the association and dissociation rates of these processes. These techniques include band-broadening measurements, the peak decay method, peak fitting methods, the split-peak method, and free fraction analysis. This review will examine the general principles and applications of these approaches and discuss their use in the characterization, screening and analysis of drug–protein interactions in the body. Graphical abstract
Source: Drug Discovery Today: Technologies - Category: Drugs & Pharmacology Source Type: research