CD4 and CD8 T cells participate in the immune memory response against  Vaccinia virus after a previous natural infection

Publication date: 2013 Source:Results in Immunology, Volume 3 Author(s): Daniela Carla Medeiros-Silva, Eduardo Augusto dos Santos Moreira-Silva, Juliana de Assis Silva Gomes, Flávio Guimarães da Fonseca, Rodrigo Correa-Oliveira The present study evaluates the immune response of memory CD4+ and CD8+ T cells from patients following a natural Vaccinia virus (VACV) infection. A total of 42 individuals were involved in the study being: 22 previously infected individuals (vaccinated or not against smallpox) and 20 non-infected individuals (vaccinated or not). A short-term in vitro stimulation with UV-inactivated VACV of whole blood cells was performed. Our study showed that previously infected individuals have a lower percentage of CD4+ T cells expressing lymph-node homing receptors (CD4+CD62L+CCR7+) and higher percentage of memory CD4+ T cells subsets (CD4+CD45ROHigh) when compared with non-infected subjects, after in vitro viral stimulation. We also showed that infected individuals presented higher percentages of CD4+ and CD8+ memory T lymphocytes expressing IFN-γ when compared to non-infected individuals. We verified that the percentage of CD4+ and CD8+ T memory cells expressing TNF-α was higher in infected and non-infected vaccinated subjects when compared with non-infected unvaccinated individual. We also observed that previously infected individuals have higher percentages of CD8+ T cells expressing lymph-node homing receptors (CCR7+ and CD62L+) and that the...
Source: Results in Immunology - Category: Allergy & Immunology Source Type: research