Diagnosis of Fasciola infection by SDS –PAGE eluted excretory secretory (ES) protein fractions using dot-ELISA

Publication date: December 2014 Source:International Journal of Veterinary Science and Medicine, Volume 2, Issue 2 Author(s): M.A. Sabry, E.S. Taher, N. Farag Allah, A.M. Mahgoub Fascioliasis is now recognized as an emerging zoonotic disease in Egypt. Diagnosis in suspected patients still needs some degree of accuracy. In the present study, three Fasciola gigantica execratory secretory (ES) protein bands of molecular weight (MW) ranging from 14 to 20kDa, 25 to 32kDa and 45 to 65kDa were eluted after fractionation of the parasite antigen using SDS–PAGE. The extracted kDa protein bands were concentrated and evaluated in diagnosis of Fasciola infection. Moreover the level of their cross reaction with other parasitic infections in infected and suspected patients of known parasite eggs/gram stool was evaluated using the dot-ELISA technique. Protein bands in the range of 14–20kDa and that of 25–32kDa were markedly specific and sensitive in diagnosis of different levels of anti-Fasciola antibodies (Ab) in sera of infected cases. These two groups of bands were able to exclude cross-reaction between anti-Fasciola Ab and other parasites recorded in stool of selected patients suffering from Schistosoma mansoni, Ascaris, and Giardia, either in single or mixed conditions with Fasciola eggs. While that of 45–65kDa appeared less specific than the other previously mentioned bands. Protein bands in the range of 25–32kDa appeared more sensitive than the other protein band...
Source: International Journal of Veterinary Science and Medicine - Category: Veterinary Research Source Type: research