Assessment of global proteome in LNCaP cells by 2D-RP/RP LC –MS/MS following sulforaphane exposure

Publication date: December 2015 Source:EuPA Open Proteomics, Volume 9 Author(s): Gregory W. Watson, Samanthi Wickramasekara, Claudia S. Maier, David E. Williams, Roderick H. Dashwood, Emily Ho The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC–MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC–MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research