Chapter 12 Bacterial Typing and Identification By Genomic Analysis of 16S –23S rRNA Intergenic Transcribed Spacer (ITS) Sequences

Publication date: 2014 Source:Methods in Microbiology, Volume 41 Author(s): Volker Gürtler, Gangavarapu Subrahmanyam, Malathi Shekar, Biswajit Maiti, Indrani Karunasagar The rrn operon is the basic building block of all bacteria found in 1–15 copies per bacterial genome in all bacteria. All bacterial genomes contain rrn operons that contain four basic components: (i) 16S rRNA gene; (ii) 16S–23S intergenic transcribed spacer (ITS); (iii) 23S rRNA gene; (iv) 5S rRNA gene. The chapter “Bacterial Typing and Identification By Genomic Analysis of 16S–23S rRNA Intergenic Transcribed Spacer (ITS) Sequences” by Volker Gürtler et al., describes the methodology used to construct a FileMaker database for analysing and presenting information about the rrn operon and how its different components relate to each other. The database, named “RiboTyping” uses a combination of graphical, text and numerical information to analyse differences between the components within and between genomes. RiboTyping uses the capabilities of FileMaker to present graphics files in multiple records as a “movie” and it summarises text information numerically by sorting specific subsets of data. This methodology enables the entire collection of thousands of bacterial genomes to be analysed at the level of multi-copy rrn operons.
Source: Methods in Microbiology - Category: Microbiology Source Type: research