Gold Nanoparticle-Based F & ouml;rster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction

Estrogen receptors play critical roles in regulating genes responsible for development and maintenance of reproductive tissues and other physiological function. The interaction of ERs with DNA sequences, known as estrogen response elements (EREs) (a palindromic repeat separated by three-base spacer, 5 & prime;GGTCAnnnTGACC-3 & prime;), is required for estrogen regulation of target gene expression. Here, we describe a simple & ldquo;mix-and-measure & rdquo;-based method for detecting ER:ERE interactions using ERE-immobilized metal nanoparticles and water-soluble conjugated polyelectrolytes (CPEs) as cooperative sensing elements. This method can differentiate the distinct DNA-binding affinity between ER & alpha; and ER & beta;, and determine ER:ERE-binding stoichiometry. This method can also accurately detect all 15 singly mutated EREs (i.e., three possible base substitutions at each of one to five positions from left to right of the 5 & prime; end half site, GGTCA) for their binding energy to ER. This method is compatible with 96-well plate format for high-throughput analysis.
Source: Springer protocols feed by Biochemistry - Category: Biochemistry Source Type: news